WebThe LyseBlue indicator dye added to some of the buffers is Thymophthalein, pH shift from colorless to blue at pH 9.3 0 votes 0 thanks Enes Yağız Akdaş Thanks for your answer, I have fix the solution by using phenolred it is light purple in resuspention buffer and become deep purple in lysis step . During neutralisation it becomes yellow WebWith optional pH indicator allows easy determination of of optimal pH for DNA engagement to the spin column. ... such as QIAvac 24 Plus with QIAvac Luer Adapters. The QIAquick PCR Washing Kit, in addition to other QIAGEN spin-column-based kits, can be fully automated upon the QIAcube, enabling greater productivity and standardization of ...
Lab Safety Guideline: Qiagen Kits - Harvard University
WebMar 9, 2010 · The MiniElute® PCR Purfication Kit from Qiagen is available in two different sizes, for 50 or 250 purifications, to isolate DNA sizes ranging from 70 bp- 4 kb. The kit comes with spin columns with silica membranes, collection tubes, buffers and pH indicator. A detailed protocol for performing the isolation using a microcentrifuge or ... WebProduct Details. The QIAquick Gel Extraction Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of DNA fragments from gels (up to 400 mg slices) or enzymatic reactions. DNA ranging from 70 bp to 10 kb is purified using a simple and fast bind-wash-elute procedure and an elution volume of 30–50 μl. princeton elementary school website
QIAGEN Plasmid Purification Handbook - Harvard University
WebG2 (Digestion buffer): 800 mM GU-HCl 30 mM Tris-HCl pH 8.0 30 mM EDTA pH 8.0 5% Tween-20 5% Triton-X100 Y1 (Yeast lysis Buffer): 40ºC storage 1 M Sorbitol 100 mM EDTA pH 8.0 14 mM beta mercaptoethanol (added just before use) LyseBlue® (pH indicator dye, 1000x): pH shift from colorless to blue at pH 9.3; 43 mg/ml Thymophthalein in 100% ethanol WebAdd 1:250 volume pH indicator I to Buffer PB (i.e., add 120 µl pH indicator I to 30 ml Buffer PB or add 600 µl pH indicator I to 150 ml Buffer PB). The yellow color of Buffer PB with … WebThe QIAGEN App is a great resource for iPhone and iPad users that helps to ease and simplify your daily work in the lab. The app provides simultaneous multiple lab timers, … princeton elementary school michigan