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Gene-indexed mutations in maize

WebThe availability of the B73 inbred reference genome sets the stage for high-throughput functional characterization of maize genes on a whole-genome scale. Among the 39 324 … WebThis gene-indexed genome-wide mutation collection provides an important resource for functional analysis of maize genes and may bring desirable allelic variants for …

Molecular Characterization of Resistance to Nicosulfuron in

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Cloning and functional analysis of GhDFR1, a key gene of …

WebWe have generated a gene-indexed maize mutant collection through ethyl methanesulfonate mutagenesis and detected the mutations by combining exome capture and next-generation sequencing. A total of 1086 mutated M plants were sequenced, and 195 268 CG>TA-type point mutations, including stop gain/loss, missplice, start … WebThe distribution of mutations was generated with a generic gene model that has the same number of exons and introns, and ratio between coding and noncoding sequences as the ACCase gene. Mutations in introns and noncoding regions were considered to be neutral, while exons had a ratio of 0.25/0.75 (neutral/deleterious) mutations according to ... WebMar 5, 2024 · This gene-indexed genome-wide mutation collection provides an important resource for functional analysis of maize genes and may bring desirable allelic variants for genetic breeding in maize. Keywords: ethyl methanesulfonate mutation; … bulky collection

Gene-Indexed Mutations in Maize ... - Molecular Plant

Category:中国农业大学生物学院 科研进展 【植物方向】学院近期高水平论 …

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Gene-indexed mutations in maize

Mapping-by-Sequencing via MutMap Identifies a Mutation in

WebMay 9, 2007 · In maize, FSTs have been generated using DNA transposons. Transposable elements can generate unstable insertions that are difficult to analyze for simple knockout phenotypes. Transposons can also generate somatic insertions that fail to segregate in subsequent generations. Results WebApr 12, 2024 · In recent years, maize growers from Northeast China have complained about unsatisfactory control of this weed after nicosulfuron treatment. The objectives of this study are to (1) identify the target-site basis of the possible presence of a point mutation in the ALS gene and (2) determine the NTSR and identify candidate genes in S. viridis.

Gene-indexed mutations in maize

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WebOct 21, 2024 · Here, we generated a new sequence-indexed maize Mutator insertional library named ChinaMu through high-throughput sequencing of enriched Mu -tagged sequences. A total of 2,581 Mu F2 lines were analyzed, and 311,924 nonredundant Mu insertion sites were obtained. WebWe have generated a gene-indexed maize mutant collection through ethyl methanesulfonate mutagenesis and detected the mutations by combining exome capture and next-generation sequencing. A total of 1086 mutated M 1 plants were sequenced, and 195 268 CG > TA-type point mutations, including stop gain/loss, missplice, start …

WebOct 27, 2011 · drove maize domestication. A new study shows an inserted retroelement in the regulatory sequences of the maize tb1 gene, which controls shoot branching, was … WebWe detected an average of 180 mutations per mutant line and 6.1 mutations per gene. As many as 27 214 mutations of start codons, stop codons, or missplice sites were …

WebMar 2, 2024 · In maize, several GMS genes, such as MAC1 (Wang et al. 2012 ), Ms23 (Moon et al. 2013 ), and Ms32 (Nan et al. 2024 ), regulate tapetal differentiation before meiosis. Notably, mutations of many GMS gene function at postmeiosis stages exhibit apoptosis defects in tapetal cells (Lei and Liu 2024; Timofejeva et al. 2013 ). Webgenetics resources for characterizing gene knockouts in maize. Results A sample of 106 sequence-indexed insertion sites from the UniformMu population were tested for …

WebMutat Res 17: 142–144. CrossRef CAS Google Scholar. Olejniczak J (1986) The genetic variability in Maize ( Zea mays L.) induced by mutagens; effect of combined doses of N …

WebPrinciples of Geneticsis one of the most popular texts in use for the introductory course. It opens a window on the rapidly advancing science of genetics by showing exactly how genetics is done. Throughout, the authors incorporate a human emphasis and highlight the role of geneticists to keep students interested and motivated. The seventh edition has … bulky clothesWebDec 6, 2024 · Gene-Indexed Mutations in Maize Discussion. A genome-wide maize mutant collection was generated using EMS mutagenesis in combination with exome … bulky collection ipswichWebMay 9, 2007 · In maize, FSTs have been generated using DNA transposons. Transposable elements can generate unstable insertions that are difficult to analyze for simple … bulky collection doncasterWebMar 2, 2015 · However, to date, there have been no efforts to develop the Spm/En system for sequence-indexed mutation screening. Robertson’s Mutator: Robertson’s Mutator ... The effect of X-rays upon mutation of the gene A in maize. Genetics 33: 273–303. [PMC free article] [Google Scholar] Stern D. B., Hanson M. R., Barkan A., 2004. bulky collection my ipswichWebApr 11, 2024 · (2024) Optimized prime editing efficiently generates heritable mutations in maize. J Integr Plant Biol. 65(4):900-906. 7. Tian T, Wang S, Yang S, Yang Z, Liu S, Wang Y, Gao H, Zhang S, Yang X, Jiang C, Qin F. (2024) Genome assembly and genetic dissection of a prominent drought-resistant maize germplasm. Nat Genet. 55(3):496-506. bulky collection doncaster councilWebWe developed a novel Ethyl Methanesulfonate (EMS) induced mutation resource in maize comprising 2050 independent M2 mutant families in the elite tropical maize inbred ML10. … bulky collection medwayWebNov 30, 2024 · Gene-Indexed Mutations in Maize. Abstract: The availability of the B73 inbred reference genome sets the stage for high-throughput functional characterization of maize genes on a whole-genome scale. Among the 39 324 protein-coding genes predicted, the vast majority are untapped due to the lack of suitable high-throughput reverse … bulky collection medway council