Bombesin-saporin
WebOct 30, 2015 · Although intrathecal injection of a GRPR antagonist or ablation of GRPR-expressing neurons with conjugated bombesin-saporin (fig. S6) was effective in blocking chloroquine-evoked itch (Fig. 3, D and F), these manipulations failed to blunt scratching in response to mechanical stimulation in NPY::Cre IN–ablated mice (Fig. 3, C and E). ... Webnoun. bom· be· sin ˈbäm-bə-sin. : a polypeptide that is found in the brain and gastrointestinal tract and has been shown experimentally to cause the secretion of various substances …
Bombesin-saporin
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WebItch and pain are two distinct sensations. Although our previous study suggested that gastrin-releasing peptide receptor (GRPR) is an itch-specific gene in the spinal cord, a long-standing question... WebJan 2, 2024 · Bombesin-saporin was injected 3 weeks after viral injection. And the behavioral and histological experiments were performed 10 days after bombesin-saporin or blank-saporin injection. Tamoxifen induction. For labeling the GRPR-positive neurons with fluorescence, we have crossed GRPR-iCreERT2 and Ai9 mice. To induce the Cre …
WebMice were given a single intrathecal injection of Bombesin-saporin (400 ng). Intrathal OT-induced scratching bouts was then recorded for 30 min two weeks after Bombesin … WebNov 1, 2014 · Intrathecal administration of the neurotoxin bombesin-saporin reduces or abolishes pruritogen-evoked scratching behavior. We investigated whether spinal …
WebMay 31, 2024 · Bombesin-saporin (Catalog: IT-40) and blank-saporin (Catalog: IT-21) were purchased from Advanced targeting systems. Animals. Oprm1. fl/fl (stock No: 030074), Vgat-ires-Cre (stock No: 016962), Sst-ires-Cre (stock No:013044), Trpv1-Cre (stock No:017769), Ai32 (stock No:024109), C57BL/6J (stock No:000664), NOD. CB- WebJun 20, 2012 · 0.13 µg of stable SP-saporin (SSP-saporin) or 1.3 µg of bombesin-saporin or 10 µg of blank- saporin (Advanced Targeting System, San Diego in 20µl of PBS) were administered to the lumbar 4 to 5 ...
WebCatalog Number IT-40-25. Format PBS (0.14 M Sodium Chloride; 0.003 M Potassium Chloride; 0.002 M Potassium Phosphate; 0.01 M Sodium Phosphate; pH 7.4), no …
WebMice were given a single intrathecal injection of blank-saporin (400 ng/5 µl), bombesin-saporin (200, 300, 400 ng/5 µl) or saline (5 µl). Mice were used for behavior experiments at 2 weeks after the treatment. Some mice were perfused at 2 or 4 weeks after treatment for molecular marker analysis. Itch behavior diy horseshoe boot rackWebFeb 8, 2016 · Bilateral preBötC injections of saporin conjugated with either bombesin (BBN–SAP; Advanced Targeting System; 200 nl, 6.2 ng) or a non-targeted peptide … craigslist shreveport cars trucksWebJun 13, 2024 · Immunotoxin 192-IgG-SAP (192-Saporin) 192-IgG-SAP is a chemical conjugate of the mouse monoclonal antibody 192-IgG that recognizes low affinity neurotrophin receptor and the ribosome-inactivating protein, Saporin. This anti-neuronal immunotoxin permanently eliminates cholinergic neurons of the basal forebrain (CBF), … craigslist shreveport for sale by ownerWebThus, the cellular basis of itch loss following SP-saporin or bombesin-saporin injections needs further investigation. (2) GRP and SP are expressed in both DRG and the dorsal spinal cord (Fleming et al. 2012; … diy horse teeth floatingWebMice were given a single intrathecal injection of Bombesin-saporin (400 ng). Intrathal OT-induced scratching bouts was then recorded for 30 min two weeks after Bombesin-saporin injection. Data are ... craigslist shreveport for saleWebbombesin. A 14-residue neuropeptide hormone produced in the gastric antrum that stimulates gastrointestinal (GI) smooth muscle contraction and the release of gastric acid … diy horse theme birthday partyWebAug 8, 2009 · Chen's team injected the spinal cords of mice with a neurotoxin called bombesin-saporin. It bound to GRPR and killed the neurons where the gene was expressed. When these mice then were exposed to ... craigslist shreveport personals